By Mark H. Moss MD, Randy J. Horwitz MD, PhD, Robert F. Lemanske Jr. MD (auth.), Phil Lieberman MD, John A. Anderson MD (eds.)
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Additional info for Allergic Diseases: Diagnosis and Treatment
Van Cauwenberge PB, Ingels KJAO. Rhinitis: The spectrum of the disease. In: Busse WW, Holgate ST, eds. Asthma and Rhinitis, 1995, Skokie, IL: Rand McNally, pp. 7-12. 3 Diagnostic Tests in Allergy Dennis R. Ownby, MD CONTENTS INTRODUCTION SKIN TESTING FOR DETECTION OF ALLERGEN-SPECIFIC laB MEASUREMENT OF ALLERGEN-SPECIFIC 1GB TOTAL SERUM laB THE FUTURE OF ALLERGY TESTING SUGGESTED READING INTRODUCTION Many physicians have the mistaken impression that allergic diseases are diagnosed by allergy tests.
Specific antibodies bind to the allergen, and nonbound antibodies are removed by washing. (C) Labeled antihuman IgE antibody is incubated with the solid phase, and the anti-IgE antibody binds to the immobilized IgE. Nonbound anti-IgE is washed away. (D) The amount of anti-lgE antibody on the solid phase is proportional to the concentration of allergen-specific IgE in the serum tested. anti-IgE antibodies and was called the radio allergo sorbent test or RAST. Because of its initial market dominance the term RAST is often used as a generic term to mean any test for allergen-specific IgE antibodies.
With adequate glucocorticoid therapy, total serum IgE levels fall. A sudden increase in serum IgE may herald disease exacerbation and allow time to alter therapy before symptoms increase or more lung damage occurs. There are other conditions in which total serum levels of IgE may be abnormal. Among the more common nonallergic causes of elevated serum IgE are metazoan parasitic infections, smoking, and AIDS. IgE is grossly elevated in the rare cases of IgE myelomas that have been reported, but the levels of IgE may still be too low to be detected as a monoclonal spike on serum protein electrophoresis.
Allergic Diseases: Diagnosis and Treatment by Mark H. Moss MD, Randy J. Horwitz MD, PhD, Robert F. Lemanske Jr. MD (auth.), Phil Lieberman MD, John A. Anderson MD (eds.)