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ISBN-10: 1483231615

ISBN-13: 9781483231617

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Glutamate may be injected into the cerebrospinal fluid or into the blood, the resulting experimental distribution of the label being quite different in the two cases), or it may even be perturbed simultaneously with labeling, as by ammonia intoxication. The resulting data may then be simulated, taking from the literature known chem­ ical concentrations, enzyme profiles, etc. , there appears to be a close association of transaminase with some enzyme capable of removing the amide group of glutamine equilibrates with a-ketoglutarate much faster than it could if glutamate formed in the process had to equilibrate with the glutamate pool).

It is well-known that on adding glucose to ascites cells in the endogenous state, there is an immediate pile-up of glucose-6-phosphate (G-6-P) that falls again and subsequently rises; ADP rises and falls while ATP inversely falls and rises; AMP follows ADP, but with smaller amplitude of change. The rate of glucose uptake is fairly constant for some time, then slows down quite drastic­ ally, and remains fairly constant at this lower level there­ after. These changes begin within a second of the glucose addition, and continue for 30 to 60 seconds (depending on the temperature).

Since the flux through all reaction steps must be the same in the stationary state (for this type of system), a line is drawn through the v Q. The intersection of this line with the other dynamical curves determines the values of the dynamical variables, x^ (as indicated for x j ) . It may be noted that this simple system of sequential reactions is extremely well behaved. Any value of Xq or vo leads to an intersection with all the dynamical characteris­ tics of the other intermediates. Since the stationary state flux (v) is entirely determined by x Q, and its dynamical char­ acteristic, the stationary state character of the net reaction is then Q [SJ - - ^ P V o m F" k o o A second example involving a sequence of simple enzyme reactions is illustrated In Figure 12.

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Control of Energy Metabolism

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